Gene Silencing of Tead3 Abrogates Radiation-induced Adaptive Response in Cultured Mouse Limb Bud Cells
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- VARES Guillaume
- National Institute of Radiological Sciences
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- WANG Bing
- National Institute of Radiological Sciences
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- TANAKA Kaoru
- National Institute of Radiological Sciences
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- SHANG Yi
- National Institute of Radiological Sciences
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- TAKI Keiko
- Clinical Genetic Oncology, Cancer Institute Hospital, Japanese Foundation for Cancer Research
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- NAKAJIMA Tetsuo
- National Institute of Radiological Sciences
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- NENOI Mitsuru
- National Institute of Radiological Sciences
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抄録
There is a crucial need to better understand the effects of low-doses of ionizing radiation in fetal models. Radiation-induced adaptive response (AR) was described in mouse embryos pre-exposed in utero to low-doses of X-rays, which exhibited lower apoptotic levels in the limb bud. We previously described AR-specific gene modulations in the mouse embryo. In this study, we evaluated the role of three candidate genes in the apoptotic AR in a micromass culture of limb bud cells: Csf1, Cacna1a and Tead3. Gene silencing of these three genes abrogated AR. Knowing that TEAD3 protein levels are significantly higher in adapted cells and that YAP/TAZ/TEAD are involved in the control of cell proliferation and apoptosis, we suggest that modulation of Tead3 could play a role in the induction of AR in our model, seen as a reduction of radiation-induced apoptosis and a stimulation of proliferation and differentiation in limb bud cells.
収録刊行物
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- Journal of Radiation Research
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Journal of Radiation Research 52 (1), 39-46, 2011
Journal of Radiation Research 編集委員会
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詳細情報 詳細情報について
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- CRID
- 1390282680194111872
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- NII論文ID
- 10027418188
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- NII書誌ID
- AA00705792
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- ISSN
- 13499157
- 04493060
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- NDL書誌ID
- 10953719
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可