An Approach to Estimate Radioadaptation from DSB Repair Efficiency

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  • YATAGAI Fumio
    Metallomics Imaging Research Unit, Center for Molecular Imaging Science, The Institute of Physical and Chemical Research (RIKEN)
  • SUGASAWA Kaoru
    Biosignal Research Center, Kobe University
  • ENOMOTO Shuichi
    Metallomics Imaging Research Unit, Center for Molecular Imaging Science, The Institute of Physical and Chemical Research (RIKEN)
  • HONMA Masamitsu
    Division of Genetics and Mutagenesis, National Institute of Health Sciences

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In this review, we would like to introduce a unique approach for the estimation of radioadaptation. Recently, we proposed a new methodology for evaluating the repair efficiency of DNA double-strand breaks (DSB) using a model system. The model system can trace the fate of a single DSB, which is introduced within intron 4 of the TK gene on chromosome 17 in human lymphoblastoid TK6 cells by the expression of restriction enzyme I-SceI. This methodology was first applied to examine whether repair of the DSB (at the I-SceI site) can be influenced by low-dose, low-dose rate gamma-ray irradiation. We found that such low-dose IR exposure could enhance the activity of DSB repair through homologous recombination (HR). HR activity was also enhanced due to the pre-IR irradiation under the established conditions for radioadaptation (50 mGy X-ray-6 h-I-SceI treatment). Therefore, radioadaptation might account for the reduced frequency of homozygous loss of heterozygosity (LOH) events observed in our previous experiment (50 mGy X-ray-6 h-2 Gy X-ray). We suggest that the present evaluation of DSB repair using this I-SceI system, may contribute to our overall understanding of radioadaptation.<br><br>Translated and modified from Radiat. Biol. Res. Comm. Vol.43(4); 443-453, (2008, in Japanese).

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