TEM/EDX Observation of Micro-beads of Magnetically Activated Cell Sorting (MACS) System and Separation Trial of Mouse Mesenchymal Stem Cells from Bone Marrow Cells by MACS System

DOI
  • TAIRA Masayuki
    Department of Dental Materials Science and Technology, Iwate Medical University School of Dentistry, Iwate, Japan
  • SASAKI Kaori
    Department of Dental Materials Science and Technology, Iwate Medical University School of Dentistry, Iwate, Japan
  • SAITOH Setsuo
    Department of Dental Materials Science and Technology, Iwate Medical University School of Dentistry, Iwate, Japan
  • NEZU Takashi
    Department of Dental Materials Science and Technology, Iwate Medical University School of Dentistry, Iwate, Japan
  • KAGIYA Tadayoshi
    Department of Oral Anatomy II, Iwate Medical University School of Dentistry, Iwate, Japan
  • HARADA Hidemitsu
    Department of Oral Anatomy II, Iwate Medical University School of Dentistry, Iwate, Japan
  • SASAKI Minoru
    Department of Oral Microbiology, Iwate Medical University School of Dentistry, Iwate, Japan
  • KIMURA Shigenobu
    Department of Oral Microbiology, Iwate Medical University School of Dentistry, Iwate, Japan
  • HIRATAS Isao
    Department of Biomaterials Science, Graduate School of Biomaterials Science, Hiroshima University, Hiroshima, Japan
  • ARAKI Yoshima
    Department of Dental Materials Science and Technology, Iwate Medical University School of Dentistry, Iwate, Japan

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説明

Magnetically activated cell sorting (MACS) system is one isolation technique of mesenchymal stem cells (MSC) from bone marrow cells. TEM/EDX observation revealed that its micro-beads had the size of about 0.5 to 1.0 µm and consisted of matrix and inorganic phases. The inorganic phase contained small particles of about 40 nm size, and consisted of Fe, Si, O, Cl (e.g., Fe3O4 + SiO2 + FeCl3). In MACS system, lineage (hematopoietic) cell depletion kit reduced the cell number of bone marrow cells to 1.2 %. Subsequent positive selection by CD117, anti-Sca-1, CD34 and CD44 micro-beads lead to the cell collection rates of 0.57 %, 0.10 %, 0.13 % and 0.14 %, respectively. A combination of three micro-beads for lineage cell depletion kit, CD117 and anti-Sca-1 micro-beads succeeded in reducing the cell collection rate down to 0.04 %, matching a normal existing rate of MSC in bone marrow cells (0.01 to 0.10 %).

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