Comparative Study of Tissue Affinity, Chemical Characteristics of Cultured and Natural Coral as a Bioabsorbable Scaffold
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- MATSUDA Yoshifumi
- Department of Oral Anatomy,
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- NISHIKAWA Tetsunari
- Department of Innovation in Dental Education,
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- OKAMURA Tomoharu
- Department of Oral Pathology,
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- TOMINAGA Kazuya
- Department of Oral Pathology,
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- WATO Masahiro
- Department of Oral Pathology,
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- TABATA Hajime
- Department of Periodontology,
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- UMEDA Makoto
- Department of Periodontology,
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- OKUSA Nobutaka
- Department of Forensic Dentistry,
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- IMAI Koichi
- Department of Biomaterials, Osaka Dental University, Osaka, Japan
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- TANAKA Akio
- Department of Oral Pathology,
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- TAMURA Isao
- Department of Oral Anatomy,
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<p>Coral is a porous material with rough surface, and physical strength that could be effectively used as scaffolding for bone growth. To compare the bioabsorbility and biocompatibility between cultured and natural coral, we examined the chemical characteristics and tissue affinity in vitro. The cultured and natural coral (montipora digitata) deproteinized with NaOH was used. The cultured and natural coral particles were soaked in calcium containing buffers (pH 6.0, pH 7.0, pH 8.0) for 10 days, and the calcium density in the buffer was then measured. The calcium density in a solution without these particles was measured as the control group. Human periodontal ligament fibroblast (HPLF) was multiplied. The cultured and natural coral particles were then seeded in the wells with HPLF. After 7 days of culture, the HPLF were stained with DAPI and 5-Ethynyl-2'-deoxyuridine (EdU), and the increase in cell number and S phase of the cell cycle were observed. Cells without these particles were cultured as a control. The density of calcium in the pH 6.0 buffer which soaked the cultured coral increased more than that which soaked the natural coral. On the other hand, the calcium density in the pH 8.0 seeded cultured coral was decreased compared with that which soaked the natural coral. After 7 days of culture, cells consisting of HPLF grew densely around the cultured coral particles compared with the natural coral. Thus, the cultured coral would provide a bioabsorbable and biocompatible scaffold.</p>
収録刊行物
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- Journal of Oral Tissue Engineering
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Journal of Oral Tissue Engineering 14 (3), 164-170, 2017
日本再生歯科医学会
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詳細情報 詳細情報について
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- CRID
- 1390282680202636800
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- NII論文ID
- 130006394594
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- ISSN
- 18800823
- 13489623
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可