<I>In Vitro</I> Cryopreservation of Moss Protonema (<I>Racomitrium japonicum</I>)

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  • AKITA Motomu
    School of Biology-Oriented Science and Technology, Kinki University
  • SEKI Daisuke
    School of Biology-Oriented Science and Technology, Kinki University
  • MURASE Haruhiko
    Department of Mechanical Engineering, Osaka Prefecture University

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Other Title
  • エゾスナゴケ（<I>Racomitrium japonicum</I>）原糸体の低温長期保存法
  • エゾスナゴケ(Racomitrium japonicum)原糸体の低温長期保存法
  • エゾスナゴケ(Racomitrium japonicum)ゲンシタイ ノ テイオン チョウキ ホゾンホウ
  • In Vitro Cryopreservation of Moss Protonema (Racomitrium japonicum)

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Abstract

This study investigated the conditions for cryopreservation of the moss protonema (Racomitrium japonicum). An aseptic culture of R. japonicum was established from a single spore obtained from surface-sterilized sporangia. The cultured protonema showed extremely high tolerance to freezing. Even when directly transferred to －80°C without cryoprotectants, the cells remained viable one year later, and began growing shortly after direct transfer to fresh Knop medium maintained at room temperature. The growth and coloration of newly generated protonemata was affected by medium composition before and during long-term preservation. The suggested optimal medium for cryopreservation is Knop medium supplemented with sucrose (10g L－1) and trace elements (B, Mn, Zn, I, Mo, Cu and Co), with or without benzyladenine.

Journal

  • Shokubutsu Kankyo Kogaku

    Shokubutsu Kankyo Kogaku 26 (1), 30-34, 2014

    Japanese Society of Agricultural, Biological and Environmental Engineers and Scientists

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