Effects of two formulations for overcoming oketsu on vascular function and expression patterns of plasma proteins in spontaneously diabetic rats

DOI 機関リポジトリ 機関リポジトリ (HANDLE) 被引用文献3件
  • 後藤 博三
    富山医科薬科大学和漢薬研究所漢方診断学部門
  • KIGA Chizuru
    Department of Pathogenic Biochemistry, Toyama Medical and Pharmaceutical University Toyama New Industry Organization
  • NAKAGAWA Takako
    Department of Kampo Diagnostics, Toyama Medical and Pharmaceutical University
  • KOIZUMI Keiichi
    Department of Pathogenic Biochemistry, Toyama Medical and Pharmaceutical University
  • SAKURAI Hiroaki
    Department of Pathogenic Biochemistry, Toyama Medical and Pharmaceutical University The 21st Century COE Program, Toyama Medical and Pharmaceutical University
  • SHIBAGAKI Yukari
    Bioinformatics Division, INTEC Web and Genome Informatics Corporation
  • OGAWA Kazuo
    Medical Evaluation Laboratory, Research Division, Tsumura and Co.
  • SHIBAHARA Naotoshi
    Department of Kampo Diagnostics, Toyama Medical and Pharmaceutical University
  • SHIMADA Yutaka
    The 21st Century COE Program, Toyama Medical and Pharmaceutical University Department of Japanese Oriental Medicine, Faculty of Medicine, Toyama Medical and Pharmaceutical University
  • SAIKI Ikuo
    Department of Pathogenic Biochemistry, Toyama Medical and Pharmaceutical University The 21st Century COE Program, Toyama Medical and Pharmaceutical University

この論文をさがす

説明

We investigated the effects of keishibukuryogan and tokishakuyakusan, which are representative formulations for overcoming oketsu, on vascular function and expression patterns of plasma proteins in spontaneously diabetic rats. Twenty-one- to 24-week-old male WBN/Kob rats were maintained for 18 weeks on a diabetes-accelerated feed, and received standard (diabetes-accelerating) chow containing 3% (wt/wt) keishibukuryogan or tokishakuyakusan for 25 weeks. There was no significant change in body weight or blood glucose among the groups. Acetylcholine-induced endothelium-dependent relaxation of the keishibukuryogan group significantly increased compared to that of controls. Xanthine/xanthine oxidase-induced contraction of the tokishakuyakusan group and phospholipase A2-induced contraction of the keishibukuryogan and tokishakuyakusan groups significantly decreased compared to the controls. Transit time of whole blood tended to decrease in the tokishakuyakusan group compared to controls. NO2-/NO3- in the keishibukuryogan and tokishakuyakusan groups significantly decreased compared to controls. A study using surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) demonstrated that five and eight peaks had significantly changed peak intensities in plasma of rats treated with keishibukuryogan and tokishakuyakusan, respectively, as compared to the controls. Thus, two representative formulations for overcoming oketsu with different mechanisms of action had favorable effects against vascular dysfunction. Altered plasma protein levels were commonly observed in the rats administered these two formulations. Our study using ProteinChip technology may be useful for the evaluation of the relationship between the efficacy and the profiling of plasma protein expression after administration Kampo medicines, thus leading to the understanding of “Sho” in Kampo medicine.

収録刊行物

被引用文献 (3)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ