Constitutive Activity of Inwardly Rectifying K+ Channel at Physiological [Ca]i Is Mediated by Ca2+/CaMK II Pathway in Opossum Kidney Proximal Tubule Cells
-
- Mori Yoshiaki
- Department of Physiology, Osaka Medical College
-
- Yoshida Hideyo
- Department of Physiology, Osaka Medical College
-
- Miyamoto Manabu
- Department of Physiology, Osaka Medical College
-
- Sohma Yoshiro
- Department of Physiology, Osaka Medical College
-
- Kubota Takahiro
- Department of Physiology, Osaka Medical College
Bibliographic Information
- Other Title
-
- Constitutive activity of inwardly rectifying K[+] channel at physiological 〔Ca〕i is mediated by Ca[2+]/CaMK 2 pathway in opossum kidney proximal tubule cells
- Constitutive activity of inwardly rectifying K channel at physiological Ca i is mediated by Ca 2 CaMK 2 pathway in opossum kidney proximal tubule cells
Search this article
Abstract
Using patch-clamp technique, we studied the role of the Ca2+/calmodulin kinase II (CaMK II)-mediated phosphorylation process on the K+ channel with an inward conductance of 90 pS in opossum kidney proximal tubule cells (OKPCs). The intracellular Ca2+ concentration ([Ca]i) was measured by use of the fluorescent dye fura 2. The following results were obtained: (i) In cell-attached patches, the channel activity was inhibited by a decrease in [Ca]i induced by perfusion with low Ca2+ (10−8 M), La3+ (100 μM), or EGTA/AM (100 μM) contained in the bath solution. The application of KN-62 (10 μM) or KN-93 (5 μM), inhibitors of CaMK II, also inhibited the channel activity. (ii) The membrane potential measured with nystatin-perforated patches was significantly decreased by the fall in [Ca]i induced by the perfusion with EGTA- or La3+-containing solution. Also, the application of KN-62 (10 μM) or KN-93 (5 μM) to the bath significantly decreased the membrane potential. (iii) In inside-out patches, the channel activity was significantly stimulated by the application of CaMK II (300 pM) at 10−7 M Ca2+ in the bath. Furthermore, the application of KN-62 (10 μM) to the bath significantly decreased the channel activity. Our findings show that the constitutive activity of inwardly rectifying K+ channel at physiological [Ca]i is mediated by the Ca2+/CaMK II pathway in OKPCs.<br>
Journal
-
- The Journal of Physiological Sciences
-
The Journal of Physiological Sciences 58 (3), 199-207, 2008
PHYSIOLOGICAL SOCIETY OF JAPAN
- Tweet
Details 詳細情報について
-
- CRID
- 1390282680228449408
-
- NII Article ID
- 10022598267
- 130004466925
-
- NII Book ID
- AA12129145
-
- ISSN
- 18806562
- 18806546
-
- NDL BIB ID
- 9549386
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- Abstract License Flag
- Disallowed