Mutagenicity and NO Formation from UVA Irradiated <i>N</i>-Nitrosoproline, and Characterization of Photoproduct Formed in UVA-irradiated Mixture of <i>N</i>-Nitrosoproline with 2′-Deoxyguanosine in Neutral Solution
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- Arimoto-Kobayashi Sakae
- Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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- Machida Masaki
- Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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- Sano Kayoko
- Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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- Aoyama Shuhei
- Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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- Asahi Chiharu
- Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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- Tanaka Noriko
- Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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- Okamoto Keinosuke
- Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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- Negishi Tomoe
- Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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- Kimura Sachiko
- School of Human Science and Environment, University of Hyogo
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- Suzuki Toshinori
- Faculty of Pharmaceutical Sciences, Shujitsu University
Bibliographic Information
- Other Title
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- Mutagenicity and NO Formation from UVA Irradiated N-Nitrosoproline, and Characterization of Photoproduct Formed in UVA-irradiated Mixture of N-Nitrosoproline with 2'-Deoxyguanosine in Neutral Solution
- Mutagenicity and NO Formation from UVA Irradiated N-Nitrosoproline, and Characterization of Photoproduct Formed in UVA-irradiated Mixture of N-Nitrosoproline with 2^|^prime;-Deoxyguanosine in Neutral Solution
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Description
Earlier studies in our laboratory showed that UVA-irradiated N-nitrosoproline (NPRO) could produce mutagenic compounds and strand breaks in treated DNA with the release of nitric oxide and active oxygen species. In the present study, we investigated the phosphate-dependence of photomutagenicity and solvent effects on nitric oxide release from irradiated NPRO. We also investigated the UVA-driven reaction of NPRO with 2′-deoxyguanosine (dG) in neutral solution, and identified the chemical structure of the photoproduct. A mixture of NPRO and dG dissolved in neutral solution was irradiated with UVA at 320-400 nm for 6 h, and the solution was then subjected to HPLC analysis. The photoproduct isolated from the NPRO and dG mixture in neutral buffer was identified as 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG). We propose a photoreaction mechanism involving the absorption of UVA photons by NPRO followed by UVA-driven photolysis of NPRO, and subsequent production of active oxygen radicals that generate 8-oxodG.<br>
Journal
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- Genes and Environment
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Genes and Environment 36 (2), 47-53, 2014
The Japanese Environmental Mutagen Society
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Details 詳細情報について
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- CRID
- 1390282680233604352
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- NII Article ID
- 130004943659
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- NII Book ID
- AA1212552X
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- COI
- 1:CAS:528:DC%2BC2cXht1ant7%2FP
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- ISSN
- 18807062
- 18807046
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- NDL BIB ID
- 025478658
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed