A Pilot Study for the Mutation Assay Using a High-throughput DNA Sequencer
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- Matsuda Tomonari
- Research Center for Environmental Quality Management, Kyoto University
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- Takamune Makiko
- National Institute of Health Sciences
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- Matsuda Yoko
- Research Center for Environmental Quality Management, Kyoto University
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- Yamada Masami
- National Institute of Health Sciences
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説明
We present here a mutation assay with little bias which incorporates high-throughput DNA sequencing technology. Our strategy is simple: 1) expose cells to a test compound, 2) isolate colonies, and 3) carry out whole-genome sequencing of the clones. In this pilot study, we used Salmonella typhimurium TA100 as a tester strain and successfully detected mutations induced by the mutagen 2-(2-furyl)-3-(5-nitro-2-furyl) acrylamide (AF-2). We believe that this new mutation assay will be a very useful tool in hazard assessment of chemicals.<br>
収録刊行物
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- Genes and Environment
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Genes and Environment 35 (2), 53-56, 2013
日本環境変異原学会
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詳細情報 詳細情報について
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- CRID
- 1390282680233647744
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- NII論文ID
- 10031158581
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- NII書誌ID
- AA1212552X
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- COI
- 1:CAS:528:DC%2BC3sXpvVejurk%3D
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- ISSN
- 18807062
- 18807046
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- NDL書誌ID
- 024474905
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDLサーチ
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- 使用不可