Oxidation of N-Alkyl-N-(3-carboxypropyl)nitrosamines by Iron Porphyrin and Oxidant Forms Alkylating Mutagens
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- Inami Keiko
- Faculty of Pharmaceutical Sciences, Tokyo University of Science Kyoritsu University of Pharmacy
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- Yasui Hiroki
- Faculty of Pharmaceutical Sciences, Tokyo University of Science
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- Tsugumi Hirotaka
- Kyoritsu University of Pharmacy
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- Ishikawa Satoko
- Kyoritsu University of Pharmacy Faculty of Pharmacy, Keio University
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- Mochizuki Masataka
- Faculty of Pharmaceutical Sciences, Tokyo University of Science Kyoritsu University of Pharmacy
書誌事項
- タイトル別名
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- Oxidation of <i>N</i>-Alkyl-<i>N</i>-(3-carboxypropyl)nitrosamines by Iron Porphyrin and Oxidant Forms Alkylating Mutagens
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抄録
N-Alkyl-N-(3-carboxypropyl)nitrosamines are known to selectively induce urinary bladder tumor in rats and mice. To detect DNA damage by N-alkyl-N-(3-carboxypropyl)-nitrosamines, we evaluated their mutagenicity using the Ames assay in S. typhimurium and E. coli under oxidative conditions of chemical model for cytochrome P450. The activation system consisted of 5,10,15,20-tetrakis(1-methylpyridinium-4-yl)porphyrinatoiron(III) pentachloride (4-MPy) plus an oxidant. The N-alkyl-N-(3-carboxypropyl)-nitrosamines; N-methyl-N-(3-carboxypropyl)nitrosamine (MCPN), N-ethyl-N-(3-carboxypropyl)nitrosamine (ECPN), N-propyl-N-(3-carboxypropyl)nitrosamine (PCPN), N-butyl-N-(3-carboxypropyl)nitrosamine (BCPN), were treated with 4-MPy and t-BuOOH in acetonitrile for 30 min at room temperature, the reaction mixture was extracted with dichloromethane, and the organic phase was assayed for their mutagenicity in Salmonella typhimurium TA1535 and Escherichia coli WP2 uvrA. The dichloromethane extract derived from the reaction mixture of MCPN, ECPN, PCPN or BCPN with 4-MPy plus t-BuOOH was mutagenic in both of the strains, indicating that N-alkyl-N-(3-carboxypropyl)nitrosamines were oxidized to direct-acting mutagens by the 4-MPy plus t-BuOOH. The mutagenicity of oxidized BCPN extract in S. typhimurium YG7108 was higher than that in S. typhimurium TA1535, suggesting that the mutagenicity derived from BCPN was due to DNA alkylation. Furthermore, the DNA seemed to be butylated, not 3-carboxypropylated, exerting the mutagenicity of BCPN in the presence of 4-MPy and t-BuOOH.<br>
収録刊行物
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- Genes and Environment
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Genes and Environment 35 (4), 99-104, 2013
日本環境変異原学会
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詳細情報 詳細情報について
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- CRID
- 1390282680233957504
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- NII論文ID
- 10031196751
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- NII書誌ID
- AA1212552X
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- COI
- 1:CAS:528:DC%2BC3sXhvVyhurvI
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- ISSN
- 18807062
- 18807046
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- NDL書誌ID
- 025004090
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- KAKEN
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- 抄録ライセンスフラグ
- 使用不可