-
- Kimura Aoi
- Drug Safety Research Laboratories, Shin Nippon Biomedical Laboratories, Ltd. Functional Biology and Pharmacology, Graduate School of Medical and Dental Sciences, Kagoshima University Division of Genetics and Mutagenesis, National Institute of Health Sciences
-
- Torigoe Naohiko
- Drug Safety Research Laboratories, Shin Nippon Biomedical Laboratories, Ltd.
-
- Miyata Atsuro
- Functional Biology and Pharmacology, Graduate School of Medical and Dental Sciences, Kagoshima University
-
- Honma Masamitsu
- Division of Genetics and Mutagenesis, National Institute of Health Sciences
この論文をさがす
説明
The comet assay has been widely used as a genotoxicity test in vitro/vivo for detecting initial DNA damage in individual cells. One of the difficulties of the assay is slide preparation, for which agarose top and bottom layers and a cell-containing middle layer are needed to immobilize the cells. To establish a practical methodology while maintaining sensitivity and reproducibility, we assessed a simple comet assay method with a hydrophilic slide glass (MAS-coat type, Matsunami glass Ind., Ltd.) instead of an agarose bottom layer. Ethyl methanesulfonate (EMS), mitomycin C (MMC), and N-nitroso dimethylamine (DMN) as genotoxic chemicals and triton X-100 (TRX) as a non-genotoxic chemical were used for validation of this method. Chinese hamster lung (CHL) cells were used. The results showed that EMS and DMN induced a significant increase in tail intensity. However, MMC, a known interstrand cross-linker, did not increase tail intensity, and it was considered that this was because MMC-induced DNA-DNA crosslinks prevent separation of the DNA duplex. TRX did not increase tail intensity. These results are consistent with previous reports and demonstrate that the simple comet assay can clearly detect genotoxicity of chemicals other than interstrand cross-linkers.<br>
収録刊行物
-
- Genes and Environment
-
Genes and Environment 32 (3), 61-65, 2010
日本環境変異原ゲノム学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390282680234260096
-
- NII論文ID
- 130004481183
- 10026643659
-
- NII書誌ID
- AA1212552X
-
- ISSN
- 18807062
- 18807046
-
- NDL書誌ID
- 10810062
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDLサーチ
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可
