Collagen vitrigel membrane: a powerful tool for skin regeneration

DOI 1 Citations 32 References
  • Aoki Shigehisa
    Department of Pathology & Microbiology, Faculty of Medicine, Saga University, Saga, Japan
  • Takezawa Toshiaki
    Transgenic Animal Research Center, National Institute of Agrobiological Sciences, Ibaraki, Japan
  • Miyazaki-Oshikata Ayumi
    Transgenic Animal Research Center, National Institute of Agrobiological Sciences, Ibaraki, Japan
  • Ikeda Satoshi
    Department of Pathology & Microbiology, Faculty of Medicine, Saga University, Saga, Japan
  • Nagase Kotaro
    Department of Dermatology, Faculty of Medicine, Saga University, Saga, Japan
  • Koba Shinichii
    Department of Dermatology, Faculty of Medicine, Saga University, Saga, Japan
  • Inoue Takuya
    Department of Dermatology, Faculty of Medicine, Saga University, Saga, Japan
  • Uchihashi Kazuyoshi
    Department of Pathology & Microbiology, Faculty of Medicine, Saga University, Saga, Japan
  • Nishijima-Matsunobu Aki
    Department of Pathology & Microbiology, Faculty of Medicine, Saga University, Saga, Japan
  • Kakihara Nahoko
    Department of Basic science of Nursing, Faculty of Medicine, Saga University, Saga, Japan
  • Hirayama Hiroshi
    Yutoku Pharmaceutical Ind. Co., Ltd, Saga, Japan
  • Narisawa Yutaka
    Department of Dermatology, Faculty of Medicine, Saga University, Saga, Japan
  • Toda Shuji
    Department of Pathology & Microbiology, Faculty of Medicine, Saga University, Saga, Japan

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Abstract

Severe burn patients lose wide areas of their skin and are confronted with a high risk of death because they lose an indispensable barrier against the invader, which in turn promotes water evaporation. Cultured skin is expected to be a promising technology for extensive skin defect patients, while faultless cultured skin has not been established. A skin sheet, tough and durable enough in clinical use, is urgently needed because typical cell sheets available now are fragile and difficult to handle at the time of surgery. Collagen vitrigel membrane is a novel biomaterial consisting of high-density collagen fibrils equivalent to connective tissues in vivo. With this novel collagen material utilized as a scaffold, we established a novel cultured skin sheet composed of keratinocytes and mesenchymal cell types. This cultured skin showed a fully differentiated epidermal layer, and could be handled with a tweezers. Interestingly, transplantation of a skin sheet revealed that acceleration of healing or inhibition against scarring depended on mesenchymal cell types in the skin sheet. Our findings suggest that the cultured skin sheet utilizing collagen vitrigel membrane cultured with keratinocytes and mesenchymal cell types will be a powerful tool for the wide skin defect and injury.

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