Establishment of a method for analyzing translesion synthesis across a single bulky adduct in human cells
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- Sawai Tomoko
- Environmental Genetics Laboratory, Frontier Science Innovation Center and Department of Biology, Graduate School of Science, Osaka Prefecture University
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- Kawanishi Masanobu
- Environmental Genetics Laboratory, Frontier Science Innovation Center and Department of Biology, Graduate School of Science, Osaka Prefecture University
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- Takamura-Enya Takeji
- Department of Applied Chemistry, Kanagawa Institute of Technology
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- Yagi Takashi
- Environmental Genetics Laboratory, Frontier Science Innovation Center and Department of Biology, Graduate School of Science, Osaka Prefecture University
書誌事項
- タイトル別名
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- Establishment of a Method for Analyzing Translesion DNA Synthesis across a Single Bulky Adduct in Human Cells
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説明
Translesion DNA synthesis (TLS) is a tolerance pathway of replication block caused by DNA lesions. To measure the efficiency and fidelity of TLS in human cells, we established a shuttle vector assay by modifying of a bacterial TLS assay system. The assay consists of transfection of DNA repair-deficient human cells with a plasmid possessing a single DNA adduct, and transformation of indicator bacteria with plasmids extracted from the cells. We show that plasmid replication was suppressed to 1/9 by a single aminobiphenyl-dG adduct, and the mutant frequency of TLS-operated plasmids was 0.31, of which the major mutation (78%) was G to T transversion. The results demonstrate that this assay is applicable in practice for investigating TLS in human cells.<br>
収録刊行物
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- Genes and Environment
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Genes and Environment 31 (1), 24-30, 2009
日本環境変異原学会
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詳細情報 詳細情報について
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- CRID
- 1390282680234492928
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- NII論文ID
- 110007138016
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- NII書誌ID
- AA1212552X
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- COI
- 1:CAS:528:DC%2BD1MXksF2gtr0%3D
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- ISSN
- 18807062
- 18807046
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- NDL書誌ID
- 10167216
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可