Somatic Embryogenesis from Cell Suspension Cultures of Taranoki (Aralia elata)

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  • KARIM Ziaul
    United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology Faculty of Agriculture, Utsunomiya University
  • YOKOTA Shinso
    Faculty of Agriculture, Utsunomiya University
  • RAHMAN Mahabubur
    United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology Faculty of Agriculture, Utsunomiya University
  • EIZAWA Junji
    Faculty of Agriculture, Utsunomiya University
  • SAITO Yasuno
    Faculty of Agriculture, Utsunomiya University
  • AZAD Mustafa Abul Kalam
    Faculty of Agriculture, Utsunomiya University
  • ISHIGURI Futoshi
    Faculty of Agriculture, Utsunomiya University
  • IIZUKA Kazuya
    Faculty of Agriculture, Utsunomiya University
  • YOSHIZAWA Nobuo
    Faculty of Agriculture, Utsunomiya University

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Abstract

The objective of this study was to increase the formation rate of embryos from suspension cultures of Taranoki (Aralia elata). The suspension cultures were prepared from leaf-derived calli. Of the various culture durations, 30 days of culture yielded the best growth of cell suspension cultures in a BT liquid medium supplemented with 2,4-D. Somatic embryogenesis has never been observed without the addition of exogenous plant growth regulators (PGRs). The highest percentage of somatic embryo formation (93.3%) was observed in a BT liquid medium supplemented with 1.0 μM BAP+5.0 μM 2,4-D. The PGR-free semi-solid BT medium showed the highest regeneration frequency of cotyledonary-shaped embryos (100%).

Journal

  • Environment Control in Biology

    Environment Control in Biology 45 (3), 173-178, 2007

    Japanese Society of Agricultural, Biological and Environmental Engineers and Scientists

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