Fundamental examination of immunological and chemical methods for detecting human and animal hemoglobin in the fecal occult blood test

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  • ヒトヘモグロビンと動物ヘモグロビンに対する便潜血検査免疫法と化学法の基礎的検討
  • ヒトヘモグロビン ト ドウブツ ヘモグロビン ニ タイスル ベンセンケツ ケンサ メンエキホウ ト カガクホウ ノ キソテキ ケントウ

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We compared the immunological and chemical methods to confirm the individual detection limits of various animal hemoglobins in the fecal occult blood test by using the hemolyzed hemoglobin solution and hemoglobin added artificial feces. The hemolyzed hemoglobin solution showed that the immunological method is highly specific and quantitative for human hemoglobin, but the chemical method has reactivity not only for human, but also animal hemoglobin. The hemoglobin added artificial feces showed that there is no apparent difference in the detection limits between the immunological method and the chemical method. In addition, the immunological method showed about 2% cross-reactivity with the bovine hemoglobin by both assays of the hemoglobin solution and hemoglobin added artificial feces. <BR>The immunological method never attained the positive result of the assay in the animal hemoglobins even at a high concentration of 1000mcg/g artificial feces.

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