Observation of fluctuation of actin filaments in living cell

DOI
  • Oka Tomoteru
    Department of Mechanical Engineering, College of Engineering, Nihon University
  • Kataoka Noriyuki
    Department of Mechanical Engineering, College of Engineering, Nihon University

Bibliographic Information

Other Title
  • 生細胞内アクチンフィラメントの微小ゆらぎの計測

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Description

<p>Actin filaments play a major role for maintain the cell morphology and sensing mechanical stresses in many types of cell. It is well known that cytoskeletal structure and proteins in and on cells are fluctuating with the thermal effect. Here, we have observed very small fluctuation of actin filaments in living cell. Actin filaments in NIH3T3 were visualized with Lifeact-GFP fusion protein. The fluorescent images were taken with the forcal microscopy 10 images every second. From the sequential images, we analyzed the width and frequency of actin fluctuation. The frequency of actin fluctuation was about 2.3 Hz and the width was 0.3 μm. It is possible that these values are affected with mechanical conditions, such as flow and the change of temperature. We continue to analyze the actin fluctuation under several types of mechanical conditions.</p>

Journal

Details 詳細情報について

  • CRID
    1390282680246892544
  • NII Article ID
    130006077164
  • DOI
    10.11239/jsmbe.55annual.433
  • ISSN
    18814379
    1347443X
  • Text Lang
    ja
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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