A CASE OF SUSPECTED PARTIAL D BECAUSE OF WEAK REACTIVITY TO ANTI-D ON COLUMN AGGLUTINATION TECHNOLOGY AND IDENTIFIED AS PARTIAL D (DBT-1) BY GENETIC TESTING
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- Nishiyama Yukari
- Department of Laboratory Medicine, Fukuoka University Hospital
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- Izumida Kumiko
- Department of Laboratory Medicine, Fukuoka University Hospital
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- Kinoshita Misae
- Department of Laboratory Medicine, Fukuoka University Hospital
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- Furuya Tomoko
- Department of Laboratory Medicine, Fukuoka University Hospital
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- Yoshiura Yoko
- Division of Transfusion Medicine, Fukuoka University Hospital
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- Kawashima Hironobu
- Department of Laboratory Medicine, Fukuoka University Hospital
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- Matsunaga Akira
- Department of Laboratory Medicine, Fukuoka University Hospital
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- Ideguchi Hiroshi
- Division of Transfusion Medicine, Fukuoka University Hospital
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- Takubo Tomoko
- Japanese Red Cross Kyushu Blood Center
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- Sakota Iwane
- Japanese Red Cross Kyushu Blood Center
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- Tomonari Yoko
- Japanese Red Cross Kyushu Blood Center
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- Sato Hiroyuki
- Japanese Red Cross Kyushu Blood Center
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- Kiyokawa Hiroyuki
- Japanese Red Cross Kyushu Blood Center
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- Tanaka Mitsunobu
- Japanese Red Cross Osaka Blood Center
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- Takahashi Junko
- Japanese Red Cross Osaka Blood Center
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- Tani Yoshihiko
- Japanese Red Cross Osaka Blood Center
Bibliographic Information
- Other Title
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- カラム凝集法での抗Ｄ反応が弱いためｐａｒｔｉａｌ Ｄを疑い，遺伝子検査でｐａｒｔｉａｌ Ｄ（ＤＢＴ－１）と判明した１例
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Description
A 58-year-old man was admitted to our hospital because of cerebral hemorrhage. Blood type testing on admission with the automated instrument AutoVue Innova® showed weak reactivity to anti-D, indicating the possibility of weak D or partial D. Further examinations using anti-D polyclonal and monoclonal antibodies showed a similar reactivity pattern as for the partial D category DBT phenotype.<br> The genomic DNA analysis by polymerase chain reaction with RHD exon-specific primers revealed no amplification of exons 5 to 7, and the following cDNA analysis suggested exons 5 to 7 of the RHD gene were replaced by RHCE equivalents. Thus, the patient was identified as DBT-1 (RHD-CE (5-7)-D).<br> Reactivity of the anti-D by column agglutination technology is generally strong compared to that of the tube method. It is therefore important to keep in mind the possibility of weak D or partial D if the reactivity of the anti-D by column agglutination technology is weaker than 3+.<br>
Journal
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- Japanese Journal of Transfusion and Cell Therapy
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Japanese Journal of Transfusion and Cell Therapy 57 (4), 267-273, 2011
The Japan Society of Transfusion Medicine and Cell Therapy
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Details 詳細情報について
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- CRID
- 1390282680247537920
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- NII Article ID
- 130004542634
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- ISSN
- 18830625
- 18813011
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- Text Lang
- ja
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed
