INTER-LABORATORY COMPARISON OF CD34<sup>+</sup> CELL ENUMERATION

DOI Web Site 3 Citations 10 References Open Access
  • Haraguchi Kyoko
    Division of Transfusion and Cell Therapy, Tokyo Metropolitan Cancer and Infectious Diseases Center Komagome Hospital
  • Okuyama Yoshiki
    Division of Transfusion and Cell Therapy, Tokyo Metropolitan Cancer and Infectious Diseases Center Komagome Hospital
  • Tanosaki Ryuji
    Department of Blood Transfusion and Cellular Therapy, National Cancer Center Hospital
  • Kunitomo Yukiko
    Division of Transfusion and Cell Therapy, Tokyo Metropolitan Cancer and Infectious Diseases Center Komagome Hospital
  • Yoshida Shigeyoshi
    Department of Clinical Laboratories, National Cancer Center Hospital
  • Uemura Tomoe
    Department of Transfusion and Cell Therapy, Keio University Hospital
  • Fukawa Masanori
    Department of Transfusion Medicine, Toranomon Hospital
  • Ito Miyuki
    Japanese Red Cross Kanto-koshinetsu Block Blood Center
  • Mori Takehiko
    Division of Hematology, Department of Medicine, Keio University School of Medicine
  • Ohashi Kazuteru
    Hematology Division, Tokyo Metropolitan Cancer and Infectious Diseases Center Komagome Hospital
  • Taniguchi Shuichi
    Department of Hematology, Toranomon Hospital
  • Makino Shigeyoshi
    Department of Transfusion Medicine, Toranomon Hospital
  • Takanashi Minoko
    Japanese Red Cross Society, Blood Service Headquarters
  • Sakamaki Hisashi
    Hematology Division, Tokyo Metropolitan Cancer and Infectious Diseases Center Komagome Hospital

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Other Title
  • CD34陽性細胞測定における施設間差の検討

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Accurate enumeration of CD34+ cells is crucial for peripheral blood stem cell transplantation. However, there is currently no standardization or external quality control of CD34+ cell enumeration technics in Japan. To assess data variability among laboratories, we quantified CD34+ cells isolated from the same fresh peripheral blood stem cell samples at six expert laboratories on the same day that they were harvested from patients. The coefficient of variation was less than 25%, and data from all laboratories were closely correlated. However, data obtained from one laboratory were significantly lower than data from other laboratories. Inappropriate methods of erythrocyte lysis and cell washing-rather than non-ISHAGE gating or dual platform methods-may have caused these significantly lower counts. However, expected results were obtained when fixed control cells were enumerated. External quality assessment using appropriate samples and standardization of methodologies for CD34+ enumeration are important for reducing inter-laboratory variation in results.

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