cDNA cloning of Nephila clavata dragline silk (MaSp1) gene and comparison with the sequence of Bombyx mori fibroin heavy chain

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  • ジョロウグモ（<i>Nephila clavata</i>）牽引糸タンパク質MaSp1遺伝子のcDNAクローニングおよびカイコフィブロイン遺伝子との配列解析
  • ジョロウグモ(Nephila clavata)牽引糸タンパク質MaSpl遺伝子のcDNAクローニングおよびカイコフィブロイン遺伝子との配列解析
  • ジョロウグモ Nephila clavata ケンインシ タンパクシツ MaSpl イデンシ ノ cDNA クローニング オヨビ カイコフィブロイン イデンシ トノ ハイレツ カイセキ

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Abstract

Major ampullate silk, also known as dragline silk, from spiders is one of the strongest biomaterials known. The major ampullate spidroin 1 (MaSp1) of the Japanese golden orb-web spider, Nephila clavata, has never been characterized. Here we report the sequence of the C-terminal region of MaSp1 from N. clavata. We have cloned a partial cDNA of 905 bp length encoding MaSp1. The deduced amino acid sequence from the partial cDNA contained the repetitive sequence of 165 amino acids and the non-repetitive sequence of 99 amino acids toward the C-terminus. This repetitive sequence consisted of three amino acid motifs, An, (GA)n and GGX. This repetitive region had an amino acid composition similar to the crystalline region of Bombyx mori fibroin H with two differences: low Ser content and high Gln content in MaSp1. In contrast to the crystalline region of fibroin H that is present in hydrophobic parts, the repetitive region of MaSp1 shows an oscillating pattern of hydrophobic and hydrophilic parts. The N. clavata dragline silk had an ultimate tensile strength (UTS) of 1300MPa. The UTS obtained in the N. clavata dragline silk is significantly higher than that in the silkworm silk. The quality of Bombyx silk would be improved by replacing fibroin H with MaSp1 or by making a mixture of fibroin H and MaSp1 in the transgenic silkworm that spin spider silk. <br>

Journal

  • Sanshi-Konchu Biotec

    Sanshi-Konchu Biotec 77 (1), 1_39-1_46, 2008

    The Japanese Society of Sericultural Science

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