Detection of double stranded RNA elements in cultivated Lentinula edodes

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  • 栽培シイタケにおける2本鎖RNA因子の検出
  • サイバイ シイタケ ニ オケル 2ホン サ RNA インシ ノ ケンシュツ

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Abstract

From bag-culture of sawdust-based cultivation of Lentinula edodes, 17 substrates considered to be abnormal were collected. These substrates were classified into the following symptoms: browning defect (4 strains), white mycelial mat on the surface (8 strains), and abnormal hard knot on the surface (5 strains). In addition to the 17 strains isolated from these abnormal substrates, we examined 29 normal strains from our laboratory stock. Cultured mycelium was ground in phosphate buffer and virus particles were precipitated by adding polyethylene glycol 8000 and NaCl. Following phenol extraction of the virus particles, double-stranded RNA (dsRNA) was detected in 2 strains by agarose gel electrophoresis; one was from a normal strain and the other was from an abnormal strain with browning defect.

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