Angiotensin-converting enzyme inhibitor in water extracts from the fruiting body of Ganoderma lucidum

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  • KUMAKURA Kei
    Department of Health and Nutrition, Faculty of Health and Welfare, Takasaki University of Health and Welfare
  • OZAWA Yoshio
    Department of Health and Nutrition, Faculty of Health and Welfare, Takasaki University of Health and Welfare
  • IGARASHI Kiyohiko
    Department of Biomaterial Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • SAMEJIMA Masahiro
    Department of Biomaterial Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo

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Other Title
  • マンネンタケ子実体の水抽出物に含まれるアンギオテンシン変換酵素阻害成分
  • マンネンタケ シジツタイ ノ ミズ チュウシュツブツ ニ フクマレル アンギオテンシン ヘンカン コウソ ソガイ セイブン

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The fruiting body of Ganoderma lucidum has inhibitory activity against angiotensin-converting enzyme (ACE), which has an important role in controlling blood pressure in the human body. In the present study, we identify and characterize the ACE inhibitory compounds from water extracts of G. lucidum fruiting body. After the fractionation of ACE inhibitory compounds by ethanol, ultrafiltration, and cation-exchange column chromatography, an active fraction (named EU3C) having an IC_<50> of 89μg/mL was obtained with a yield of 14.1%. EU3C showed competitive-type inhibition on ACE activity and K_i was estimated to be 49μg/mL. Further separation of EU3C with reverse-phase chromatography led to increased IC_<50> and K_i values, suggesting that the ACE activity is inhibited by various compounds but not by a single chemical in EU3C. Protein assay and thin layer chromatography analysis based on the ninhydrin reaction revealed that EU3C includes various peptides. ACE inhibitory activity of EU3C was decreased by incubation with carboxypeptidase and acid hydrolysis. These results suggest that peptides of EU3C are responsible for the ACE inhibitory activity of G. lucidum fruiting body.

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