Screening of Chrysanthemum Plants with Strong Resistance to Chrysanthemum Stunt Viroid
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- Omori Hitomi
- Graduate School of Agriculture, Kyoto University
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- Hosokawa Munetaka
- Graduate School of Agriculture, Kyoto University
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- Shiba Hayato
- Graduate School of Agriculture, Kyoto University
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- Shitsukawa Naoki
- Department of Bioscience, Fukui Prefectural University
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- Murai Koji
- Department of Bioscience, Fukui Prefectural University
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- Yazawa Susumu
- Graduate School of Agriculture, Kyoto University
Bibliographic Information
- Other Title
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- キクわい化ウイロイドに強い抵抗性を持つキク植物体の選抜
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Abstract
Chrysanthemum stunt viroid (CSVd) is one of the problematic pathogens known to infect chrysanthemum (Dendranthema grandiflorum) plants and causes various symptoms, such as a reduction in plant height, which is a serious problem in cut flower production. No natural sources with resistance to CSVd have been reported. By quantifying the CSVd titer within the plant, we identified the cultivar ‘Utage’ as a plant in which the increase of the CSVd titer was slowest among 6 cultivars tested. ‘Utage’ self-pollinated, and 67 resulting seedlings were screened for CSVd resistance using reverse transcription polymerase chain reaction (RT-PCR), nested-PCR, micro-tissue (MT) direct RT-PCR, and real-time RT-PCR. Of these 67 seedlings, 9 plants lacked the obvious CSVd band detected by RT-PCR. Five months after grafting to CSVd-infected plants, the CSVd titers of 3 plants (C7, A30, and A27) were about 1/240, 1/41000, and 1/125000 compared to that of ‘Utage’, respectively. These 3 plants were comfirmed to have strong resistance to CSVd. In C7, local distribution of CSVd was observed in the youngest expanded leaf by micro-tissue direct RT-PCR and in situ hybridization. For A30 and A27, CSVd was hardly detected in the whole plant. These 3 plants will contribute to the elucidation of CSVd resistance mechanisms.<br>
Journal
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- Journal of the Japanese Society for Horticultural Science
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Journal of the Japanese Society for Horticultural Science 78 (3), 350-355, 2009
THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE
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Details 詳細情報について
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- CRID
- 1390282680265434368
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- NII Article ID
- 130004510684
- 10025927394
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- NII Book ID
- AA12177046
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- ISSN
- 1882336X
- 18823351
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- NDL BIB ID
- 10275627
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed