Comprehensive gene expression analysis of canine tongue mucosa and gene structure analysis of canine taste receptor families using next generation sequencer
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- Watanabe Manabu
- Laboratory of Functional Genomics, Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo
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- Chen Iris Yun-Ju
- Laboratory of Functional Genomics, Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo
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- Mori Mikako
- Laboratory of Functional Genomics, Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo
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- Ueno Emi
- Laboratory of Functional Genomics, Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo
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- Nakamura Yoshitaka
- Laboratory of Functional Genomics, Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo
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- Yabumoto Emiko
- Laboratory of Functional Genomics, Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo
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- Abe Kazumi
- Laboratory of Functional Genomics, Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo
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- Tsubaki Mari
- Laboratory of Functional Genomics, Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo
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- Imamura Kiyomi
- Laboratory of Functional Genomics, Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo
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- Sekimori Etsuko
- Laboratory of Functional Genomics, Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo
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- Nishizawa Rie
- Laboratory of Functional Genomics, Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo
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- Wakaguri Hiroyuki
- Laboratory of Functional Genomics, Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo
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- Nakagawa Takayuki
- Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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- Mochizuki Manabu
- Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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- Nishimura Ryohei
- Veterinary Medical Center, Graduate School of Agricultural and Life Sciences, The University of Tokyo Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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- Sasaki Nobuo
- Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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- Suzuki Yutaka
- Laboratory of Functional Genomics, Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo
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- Sugano Sumio
- Laboratory of Functional Genomics, Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo
Bibliographic Information
- Other Title
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- 次世代型シークエンサーを用いたイヌ舌組織由来遺伝子群の発現解析および味覚に関する遺伝子構造の解析
- ジセダイガタ シークエンサー オ モチイタ イヌ ゼツ ソシキ ユライ イデンシグン ノ ハツゲン カイセキ オヨビ ミカク ニ カンスル イデンシ コウゾウ ノ カイセキ
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Abstract
In this study, we examined the gene expression frequency of canine tongue mucosa and analyzed the gene structure of canine taste receptor-related gene families using a next-generation sequencer. First, we performed RNA-seq analysis on canine mucosa samples, obtained 984,903 short-read sequence tags, and mapped them to the canine whole-genome sequences. Gene expression analysis reveals S100A8 to be the most frequently expressed gene, while skeletal musclerelated, cardiac muscle-related, and detoxification-related genes were also found to be highly expressed. Sequence tags of the taste receptor-related genes were mapped to the 5' terminal region of TAS2R40, TRPV1, and PKD1. Based on the genomic region of TAS2R40, where the tags mapped, we deduced the primary sequence of a 306 amino-acid canine TAS2R40 protein, which shows 76% homology to the human TAS2R40, and 62% homology to the mouse TAS2R40.
Journal
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- Journal of Pet Animal Nutrition
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Journal of Pet Animal Nutrition 15 (1), 1-6, 2012
Japanese Society of Pet Animal Nutrition
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Details 詳細情報について
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- CRID
- 1390282680265517824
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- NII Article ID
- 130004565658
- 40019299268
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- NII Book ID
- AA11293143
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- ISSN
- 21857601
- 13443763
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- NDL BIB ID
- 023727758
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- CiNii Articles
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- Abstract License Flag
- Disallowed