組換え体トレニア（<i>Torenia fournieri</i> Lind.）において変異型カーネーションエチレン受容体 <i>Dc-ETR1nr</i> の発現は花弁の脱離と自己触媒的エチレン生成を抑制する
書誌事項
- タイトル別名
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- Heterologous Expression of a Mutated Carnation Ethylene Receptor Gene, Dc-ETR1nr, Suppresses Petal Abscission and Autocatalytic Ethylene Production in Transgenic Torenia fournieri Lind.
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説明
The mutated carnation ethylene receptor gene Dc-ETR1nr was introduced into Torenia fournieri Lind. This gene contains a missense mutation causing conversion of a Pro36 residue of the carnation ethylene receptor protein Dc-ETR1 to Leu36, as occurs with the tomato mutant gene Never-ripe (Nr). Agrobacterium transformation of Torenia was performed, and four putative transgenic plants with Dc-ETR1nr were obtained. Real-time RT-PCR analysis confirmed Dc-ETR1nr mRNA expression in all transgenic plants. Unlike wild-type plants and 1-aminocyclopropane-1-carboxylic acid oxidase (ACO) transgenic plants, none of the transgenic plants showed flower abscission in response to ethylene treatment. Flower life in all Dc-ETR1nr transgenic plants following wounding or ethylene treatment was longer than that in wild-type plants. Levels of autocatalytic ethylene production in all transgenic plants following wounding or pollination treatment were lower than those in wild-type plants. These results indicate that transgenic plants expressing Dc-ETR1nr have reduced ethylene sensitivity, resulting in inhibition of autocatalytic ethylene production and flower senescence.<br>
収録刊行物
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- Journal of the Japanese Society for Horticultural Science
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Journal of the Japanese Society for Horticultural Science 80 (1), 113-120, 2011
一般社団法人 園芸学会
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詳細情報 詳細情報について
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- CRID
- 1390282680266017408
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- NII論文ID
- 130004951624
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- NII書誌ID
- AA12177046
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- ISSN
- 1882336X
- 18823351
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- NDL書誌ID
- 10935073
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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- JaLC
- NDLサーチ
- Crossref
- CiNii Articles
- KAKEN
- OpenAIRE
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- 抄録ライセンスフラグ
- 使用不可
