Development of Molecular Markers Associated with Sexuality in <i>Diospyros lotus</i> L. and Their Application in <i>D. kaki</i> Thunb.
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- Akagi Takashi
- Graduate School of Agriculture, Kyoto University
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- Kajita Kei
- Graduate School of Agriculture, Kyoto University
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- Kibe Takanori
- Graduate School of Agriculture, Kyoto University
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- Morimura Haruka
- Graduate School of Agriculture, Kyoto University
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- Tsujimoto Tomoyuki
- Graduate School of Agriculture, Kyoto University
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- Nishiyama Soichiro
- Graduate School of Agriculture, Kyoto University
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- Kawai Takashi
- Graduate School of Agriculture, Kyoto University
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- Yamane Hisayo
- Graduate School of Agriculture, Kyoto University
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- Tao Ryutaro
- Graduate School of Agriculture, Kyoto University
Bibliographic Information
- Other Title
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- マメガキ（<i>Diospyros lotus</i> L.）における雌雄性判別分子マーカーの開発とカキ（<i>D. kaki</i> Thunb.）への適用
- Development of Molecular Markers Associated with Sexuality in Diospyros lotus L. and Their Application in D. kaki Thunb
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Abstract
Sexuality of crops affects both cultivation and breeding systems. Cultivated persimmon (Diospyros kaki Thunb) has a morphologically well-characterized polygamous or gyonodioecious sexual system. However, the genetic basis of sexuality in D. kaki has yet to be characterized. Here, we used dioecious D. lotus L., a diploid wild relative species to hexaploid or nonaploid D. kaki, as a model to clarify the genetic basis of sexuality in Diospyros and to develop molecular markers associated with the sexuality of individuals. Using 62 F1 offspring segregated into distinct male/female phenotypes, we found two amplified fragment-length polymorphism markers, DlSx-AF4 and DlSx-AF7, which cosegregated with maleness. This could suggest that the sexuality of D. lotus is controlled by a single gene/haploblock, and the male is dominant over the female. Thus, D. lotus’s sexuality can be described as the heterogametic male type, the XY-type, as reported for most other dioecious plant species. For unknown reasons, segregation of the phenotype of a sequence-characterized amplified region marker developed from DlSx-AF4 (DlSx-AF4S) and/or the male/female phenotype in two different crosses in D. lotus showed an apparent bias towards femaleness and better fitted 1:2 than 1:1, which is the theoretical segregation for a single genetic locus or haploblock in diploid D. lotus. DlSx-AF4S could distinguish D. kaki cultivars with female and male flowers from cultivars with only female flowers, strongly indicating that the same genetic system controls D. kaki’s sexuality and that DlSx-AF4S could be used as a genetic marker for sexuality in D. kaki breeding programs.
Journal
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- Journal of the Japanese Society for Horticultural Science
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Journal of the Japanese Society for Horticultural Science 83 (3), 214-221, 2014
THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE
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Details 詳細情報について
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- CRID
- 1390282680266094976
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- NII Article ID
- 130004510823
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- NII Book ID
- AA12177046
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- ISSN
- 1882336X
- 18823351
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- HANDLE
- 2433/191079
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- NDL BIB ID
- 025584883
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed