Simulating Hybrid-seed Contamination Risk with Selfed Seeds from Residual Fertility in a Male-sterile T-4 Mutant Tomato, Solanum lycopersicum L.
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- Masuda Masaharu
- Graduate School of Natural Science and Technology, Okayama University
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- Ojiewo Christopher Ochieng
- Graduate School of Natural Science and Technology, Okayama University
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- Nagai Mayumi
- Graduate School of Natural Science and Technology, Okayama University
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- Murakami Kenji
- Graduate School of Natural Science and Technology, Okayama University
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- Masinde Peter Wafula
- Jomo Kenyatta University of Agriculture and Technology
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- Yu Wang
- Guangxi University
Bibliographic Information
- Other Title
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- 雄性不稔突然変異トマト(T-4)を種子親に用いたときの F<sub>1</sub> 採種における自殖種子混入リスクのシミュレーション
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Abstract
Environmental dependence of male sterility may sometimes result in residual fertility under ‘sterility conditions’, causing hybrid-seed contamination risk. An experiment was conducted to assess the risk factor and methods to increase hybrid-seed purity in a thermosensitive male-sterile tomato mutant, T-4, whose fertility is partially restored in autumn, but largely remains sterile in spring, with some residual fertility. Examination of pollen germination and the subsequent pollen-tube growth in vitro and on stigma revealed that a small proportion of the T-4 pollen was viable, with 10–20% germination, while normal pollen from ‘Tiny Tim’ had 60–85% germination 3–6 h after pollination. A stable male-sterile mutant T-3, whose pollen development collapses at the microspore stage, was pollinated with T-4 pollen followed by normal ‘Tiny Tim’ pollen with time lags of 2, 4, and 8 h. Concurrently, the T-4 mutant was self-pollinated by hand followed by normal pollen from an inbred line (M) with time lags of 2, 4, 8, 24, and 48 h. The progeny was scored for contamination based on differences in leaf characteristics. The percentage of T-4 seedlings (narrow leaved) in the F1 progeny was lowest at 2 h (0.2–6.3%), highest at 8 h (16.9–17.7%) and declined at 24 h–48 h (13.5–10.3%) time lag. The contamination rate was extremely low when pollination was done with normal pollen at (0.4%) and 24 h after (1.4%) anthesis without prior hand pollination with T-4 pollen. It was concluded that with pollination timing soon after anthesis, the T-4 mutant could be effectively applied in a two-line hybrid-seed production system with lower roguing cost of undesirable seedlings as opposed to the conventional three-line system.<br>
Journal
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- Journal of the Japanese Society for Horticultural Science
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Journal of the Japanese Society for Horticultural Science 79 (1), 34-39, 2010
THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE
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Details 詳細情報について
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- CRID
- 1390282680266413440
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- NII Article ID
- 130004510693
- 40016940764
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- NII Book ID
- AA12177046
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- ISSN
- 1882336X
- 18823351
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- NDL BIB ID
- 10527024
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed