-
- スッサワト ドゥアンシイサイ
- 名古屋大学大学院生命農学研究科
-
- 山田 邦夫
- 名古屋大学大学院生命農学研究科
-
- 白武 勝裕
- 名古屋大学大学院生命農学研究科
-
- 金山 喜則
- 東北大学大学院農学研究科
-
- 山木 昭平
- 名古屋大学大学院生命農学研究科
書誌事項
- タイトル別名
-
- Properties of Sorbitol Dehydrogenase in Strawberry Fruit and Enhancement of the Activity by Fructose and Auxin
この論文をさがす
抄録
Strawberry, belonging to the Rosaceae family, translocates sucrose as the main carbohydrate to fruit, although many Rosaceae fruit trees use sorbitol as translocated sugar; however, we have found that strawberry has genes encoding sorbitol-metabolizing enzymes. In this study, the property of NAD+-dependent sorbitol dehydrogenase (NAD-SDH) and the effect of sugars and phytohormones on NAD-SDH activity and its mRNA accumulation were investigated to elucidate sorbitol metabolism and its regulation. The Km values of NAD-SDH for substrates of fructose and sorbitol were 78.7 and 7.3 mM, respectively, similar to those of maize, which synthesizes sorbitol by the reduction of fructose by NAD-SDH. This result suggested that NAD-SDH in strawberry fruit can catalyze the reduction of fructose, but the activity is not enough to accumulate sorbitol in fruit. Therefore, we investigated the effect of sugars and phytohormones on NAD-SDH activity and the transcript level by adding various sugars and phytohormones to sliced fruit discs. Tissues incubated in 100 mM fructose or sorbitol stimulated NAD-SDH activity by about 2.5-fold compared with the control. Sucrose also stimulated NAD-SDH activity, but the increase was not as high as sorbitol or fructose. Of the phytohormones treated, 100 μM indole-3-acetic acid (IAA) had a marked stimulatory effect on NAD-SDH activity, but the other phytohormones (abscisic acid, gibberellic acid and 6-benzyladenine) had no stimulative effect. The mRNA transcript level in all sugar and phytohormone treatments showed no marked increase compared with the control. Thus, the kinetics and induction profiles by phytohormones of NAD-SDH showed that sorbitol metabolism in strawberry fruit was different from that in apple and Japanese pear fruits.<br>
収録刊行物
-
- Journal of the Japanese Society for Horticultural Science
-
Journal of the Japanese Society for Horticultural Science 77 (3), 318-323, 2008
一般社団法人 園芸学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390282680266628992
-
- NII論文ID
- 110006824888
- 130004510655
-
- NII書誌ID
- AA12177046
-
- COI
- 1:CAS:528:DC%2BD1cXptVCqsL0%3D
-
- ISSN
- 1882336X
- 18823351
-
- NDL書誌ID
- 9570342
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- NDL-Digital
- CiNii Articles
- KAKEN
-
- 抄録ライセンスフラグ
- 使用不可