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- Udagawa Chihiro
- Department of Basic Science, School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University
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- Chong Yong Hwa
- Department of Basic Science, School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University
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- Shito Masayuki
- Department of Basic Science, School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University
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- Kawakami Tsuyoshi
- Department of Basic Science, School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University
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- Tada Naomi
- Department of Basic Science, School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University
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- Ochiai Kazuhiko
- Department of Basic Science, School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University
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- Ishioka Katsumi
- Department of Veterinary Nursing, School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University
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- Tsuchida Shuichi
- Laboratory of Comparative Cellular Biology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University
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- Omi Toshinori
- Department of Basic Science, School of Veterinary Nursing and Technology, Faculty of Veterinary Science, Nippon Veterinary and Life Science University
Bibliographic Information
- Other Title
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- イヌにおける<i>UCP2</i>および<i>UCP3</i> cDNAの単離と発現解析
- cDNA cloning and expression analysis of canine uncoupling protein 2 and 3 genes
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Description
The uncoupling proteins (UCPs) play an important role in energy homeostasis. To date, five UCP homologs, UCP1 to UCP5, have been identified in mammals. Both UCP2 and UCP3 have been found to decrease membrane potential and increase thermogenesis. They are regarded as inducers of obesity and type 2 diabetes in humans. We showed that the dog UCP2 and UCP3 cDNAs consisted of a part of the 5'-UTR sequence and a complete open reading frame in each gene. Comparisons with the gene structure in humans, dog genome sequences, and the transcripts in this study showed that dog UCP2 consists of exons 1 to 8 and dog UCP3 consists of exons 1 to 7. In dog UCP 3, exon 3-skipped transcripts were also observed. A UCP2 and UCP3 gene expression study using reverse transcription-polymerase chain reaction and 27 kinds of total RNA from dog tissues showed that tissue distribution of gene expression was different between dog UCP2 and UCP3. The present data may provide us with information regarding DNA polymorphism and help in further investigations of the influence of UCP2 and UCP3 activity on canine energy balance.
Journal
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- Journal of Pet Animal Nutrition
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Journal of Pet Animal Nutrition 14 (2), 68-75, 2011
Japanese Society of Pet Animal Nutrition
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Keywords
Details 詳細情報について
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- CRID
- 1390282680267089152
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- NII Article ID
- 130004565633
- 40019033797
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- NII Book ID
- AA11293143
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- ISSN
- 21857601
- 13443763
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- NDL BIB ID
- 11279844
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- CiNii Articles
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- Abstract License Flag
- Disallowed