Production of Haploid Plant of 'Banpeiyu' Pummelo [Citrus maxima (Burm.) Merr.] by Pollination with Soft X-Ray-Irradiated Pollen
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- Yahata Masaki
- Faculty of Agriculture, University of Miyazaki
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- Yasuda Kiichi
- Faculty of Agriculture, University of Miyazaki
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- Nagasawa Kohji
- School of Agriculture, Tokai University
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- Harusaki Seiichi
- School of Agriculture, Tokai University
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- Komatsu Haruki
- School of Agriculture, Tokai University
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- Kunitake Hisato
- Faculty of Agriculture, University of Miyazaki
Bibliographic Information
- Other Title
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- 軟 X 線照射花粉の受粉によるブンタン[<i>Citrus maxima</i>(Burm.)Merr.]‘晩白柚’の半数体の作出
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Description
To induce haploid plants in Citrus maxima (Burm.) Merr. ‘Banpeiyu’, we evaluated the effect of pollination with soft X-ray-irradiated pollen on fruit set and seed development, and carried out ovule culture. When ‘Banpeiyu’ pummelo pistils were pollinated with X-ray-irradiated pollen of ‘Fukuhara’ sweet orange [C. sinensis (L.) Osbeck], the exposure doses affected the fruit set. The number of seeds per fruit was also affected by the exposure dose, and tended to decrease as the dose increased; however, all developed seeds obtained from these crosses were diploid. In the ovule culture of ‘Banpeiyu’ pummelo, six embryoids shown haploidy were obtained in all treatments. One haploid plantlet with 9 chromosomes was regenerated from an embryoid in a culture of ovules established 40 days after pollination with 400 Gray (Gy)-irradiated pollen of ‘Tosa-buntan’ pummelo (C. maxima). This haploid was suggested to be derived from ‘Banpeiyu’ pummelo by random amplified polymorphic DNA (RAPD) and cleaved amplified polymorphic sequence (CAPS) analysis.<br>
Journal
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- Journal of the Japanese Society for Horticultural Science
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Journal of the Japanese Society for Horticultural Science 79 (3), 239-245, 2010
THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE
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Details 詳細情報について
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- CRID
- 1390282680267777152
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- NII Article ID
- 130004726125
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- NII Book ID
- AA12177046
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- ISSN
- 1882336X
- 18823351
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- NDL BIB ID
- 10747780
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL Search
- Crossref
- CiNii Articles
- OpenAIRE
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- Abstract License Flag
- Disallowed