Availability of Adenosine Tri-phosphate measurement method for Practical Assessment of Medical Instrumentations Cleanliness Evaluating with Fresh Human Blood

Uchida Miho, Sugawara Erisa, Kajiura Takumi, Kobayashi Hiroyoshi

doi:10.4286/jjmi.85.577

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Other Title

新鮮ヒト血液を用いたアデノシン三リン酸測定法の洗浄評価判定法の有用性に関する検討
シンセンヒトケツエキオモチイタアデノシンサンリンサンソクテイホウノセンジョウヒョウカハンテイホウノユウヨウセイニカンスルケントウ

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Abstract

The luciferin/luciferase/Adenosine Tri-Phosphate (ATP) bioluminescent method is commonly used for cleanliness verification of food processing field. Recent years measurement method using ATP is utilized in medical field for its simplicity, rapidity and highly sensitivity. However, for the assessment of the ATP measurement method has not yet showed that correlation between the protein and ATP in fresh human blood. This study examined the correlation between the amount of protein and ATP of fresh human blood. We collected blood samples from healthy 10 adults. We measured the ATP and ATP+AMP (Adenosine Mono-Phosphate) values of diluted blood solutions by using each measuring instruments. Furthermore, the amount of protein in each solution was measured by the Bradford protein assay. Both amount of ATP and ATP + AMP had greater variation and depended on the dilution obtained and mostly showed decline over time. For the amount of protein and ATP and ATP + AMP value of the log in the dilution showed strong positive correlation. The results of this study suggest the effective use of the ATP measurement method in healthcare settings.

Journal

Iryou kikigaku (The Japanese journal of medical instrumentation)

Iryou kikigaku (The Japanese journal of medical instrumentation) 85 (6), 577-582, 2016

Japanese Society of Medical Instrumentation

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