Laboratory-based surveillance of Extended-spectrum β-Lactamase-producing Escherichia coli, Klebsiella spp., and Proteus mirabilis, by the Shiga Infection Control Network

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  • NISHIO Hisaaki
    Shiga Infection Control Network Department of Clinical Laboratory, Shiga Medical Center for Adults
  • SUEYOSHI Noriyuki
    Shiga Infection Control Network Department of Clinical Laboratory, Social Insurance Shiga Hospital
  • YAMAMOTO Katsura
    Shiga Infection Control Network Kohka Public Hospital
  • FURUKAWA Akiko
    Shiga Infection Control Network Shiga University of Medical Science Hospital
  • TAKEMURA Miwa
    Shiga Infection Control Network Shiga University of Medical Science Hospital
  • YOSHIO Kuniko
    Shiga Infection Control Network Shiga University of Medical Science Hospital
  • NAKAMURA Hiroko
    Shiga Infection Control Network Nagahama City Hospital
  • INOUE Tetsuya
    Shiga Infection Control Network Department of Clinical Laboratory, Social Insurance Shiga Hospital Department of Hematology, Social Insurance Shiga Hospital

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Other Title
  • 滋賀県感染制御ネットワーク事業における耐性菌サーベイランス―基質拡張型β‐ラクタマーゼ産生グラム陰性桿菌について―
  • 滋賀県感染制御ネットワーク事業における耐性菌サーベイランス--基質拡張型β-ラクタマーゼ産生グラム陰性桿菌について
  • シガケン カンセン セイギョ ネットワーク ジギョウ ニ オケル タイセイキン サーベイランス キシツ カクチョウガタ ベータ ラクタマーゼ サンセイ グラム インセイカンキン ニ ツイテ

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  A total of 1,650 Escherichia coli, 659 Klebsiella spp., and 241 Proteus mirabilis strains were collected during a 3-month period (October-December 2006) at 32 institutes participating in the Shiga Prefecture infection control network to investigate the expression of extended-spectrum β-lactamases (ESBLs). ESBLs were detected in 55 (3.3%), 8 (1.2%), and 40 (16.6%) of the E. coli, Klebsiella spp., and P. mirabilis isolates, respectively, using the Clinical Laboratory Standards Institute Document M100-S16 and PCR analysis. Of the 55 ESBL-positive E. coli strains and 40 ESBL-positive P. mirabilis strains, 28 isolates carried the genes for CTX-M9 and 40 carried genes for CTX-M2 β-lactamases, respectively. Identical PCR fingerprint profiles were obtained by random amplified polymorphic DNA (RAPD) pattern analysis for E. coli from three separate institutes, suggesting nosocomial spread in these institutions. Moreover, P. mirabilis strains displaying identical PCR fingerprint profiles were identified from five institutes, suggesting nosocomial spread in Shiga Prefecture. Each medical facility completed a questionnaire survey, allowing us to examine infection control measures. According to the responses, the problems caused by ESBLs in facilities are understood by most facilities (29 facilities), and infection control measures were thus improved. The frequency of occurrence in the facilities and realities of ESBL diffusion in the Shiga Prefecture were clarified by this investigation. The study provided the opportunity to review the infection control measures in each facility and underlined the importance of monitoring antibiotic-resistant bacteria in the region.<br>

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