Genetic survey of genomic regions responsible for panicle spreading in wild rice, <i>Oryza rufipogon</i>

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  • 野生イネ<i>Oryza rufipogon</i>由来の穂の開帳性を支配する遺伝子領域の推定
  • Genetic survey of genomic regions responsible for panicle spreading in wild rice, Oryza rufipogon

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Description

Rice (Oryza sativa L.) is derived from the Asian common wild rice, O. rufipogon. In the early stage of domestication, panicle shape may be a key trait before the emergence of plants with non-seed shattering, because wild plants with cultivated-like type of closed panicles were observed to reduce seed shattering through seed retention. Previously, we identified a major locus for this trait to be SPR3 on chromosome 4, controlling gene expression of OsLG1. However, we also found some experimental lines, i.e., BC2F8 backcross recombinant inbred lines between O. sativa Nipponbare and O. rufipogon W630, that showed open panicles without wild alleles at SPR3. In this study, a line of AsN136 was selected from them in order to survey new genomic regions responsible for panicle spreading in wild rice. The AsN136 had wild homozygous alleles at 27 out of 180 SSR marker loci, showing nine wild chromosomal segments in the genetic background of Nipponbare. This line was crossed with Nipponbare, and the segregating population consisted of 150 BC3F2 plants were produced. They were planted in the paddy field, and their panicle shape was examined based on the observation of the rachisbranch junctions. QTL analysis for panicle shape was carried out with their marker genotype data at 27 SSR loci. As a result, two putative loci were detected on chromosomes 5 and 11, explaining 37.6% and 4.9% of the total phenotypic variance, respectively. The putative locus on chromosome 5 with stronger effect was further examined by the progeny test using BC3F3 plants. The results indicated that a new factor for panicle shape was located in the region of RM421-RM274. Expression analysis on OsLG1 and fine mapping of the new locus on chromosome 5 may give good clues to clarify the gene mechanism of panicle spreading.

Journal

  • Journal of Crop Research

    Journal of Crop Research 61 (0), 23-26, 2016

    The Society of Crop Science and Breeding in Kinki, Japan

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