書誌事項
- タイトル別名
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- Synthesis and Properties of Oligonucleotides Containing 5-Substituted Pyrimidine Nucleoside
- 5イ チカン ピリミジンヌクレオシド オ フクム オリゴヌクレオチド ノ ゴウセイ ト ソノ セイシツ
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説明
Our recent study on the oligodeoxyribonucleotide (ODN) containing 5-substituted pyrimidine nucleosides is described. 5-Substituted 2 '-deoxyuridine derivatives and 5-substituted arabinofuranosyluracil derivatives were synthesized from 2, 2 '-anhydro-5-methoxycarbonylmethyluridine, which was synthesized from arabinoaminooxazoline and dimethyl α-bromomethylfumarate. Modified ODNs bearing these nucleoside analogs were prepared chemically by pre-synthetic modification method or post-synthetic modification method. Effect of 5-substituent groups on DNA/DNA or DNA/RNA duplexes was investigated by measuring the melting behaviors. Some of these modified ODNs are expected as antisense ODNs since these could induce RNase H activity and impart stability against nuclease. Also, 5-substituted 2 '-deoxyuridine analog triphosphates served as substrates of thermophilic family B DNA polymerases in a primer extension reaction or PCR, to give the modified ODNs. 5-Methoxycarbonylmethy1-2'-deoxyuridine residues incorporated into DNA by PCR could be used to post-synthetic derivatization. This finding is useful for in vitro selection of the functionalized DNA.
収録刊行物
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- 有機合成化学協会誌
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有機合成化学協会誌 62 (12), 1238-1248, 2004
公益社団法人 有機合成化学協会
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詳細情報 詳細情報について
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- CRID
- 1390282680289885568
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- NII論文ID
- 10014119789
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- NII書誌ID
- AN0024521X
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- ISSN
- 18836526
- 00379980
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- NDL書誌ID
- 7179554
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- 本文言語コード
- ja
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- 資料種別
- journal article
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- 使用不可
