ヒト胃粘膜のthymidine-H3によるautoradiograph圧入によるincubation法について

七条 小次郎, 須藤 宏, 樋口 次男, 小林 二郎, 飯塚 春太郎, 関口 利和

doi:10.2974/kmj1951.19.79

The uptake thymidine-H³ by human gastric mucosa was studied in vitro, and the resulting labeling of cells was used as an indicator of the proliferative capacity of these tissues. As the gastric mucosa was labeled only about 100μ in depth from surface in the usual incubation technique, it is rather difficult and unreliable to examine the whole gastric mucosa. The following improved labeling technique was introduced in this paper. The gastric mucosa obtained from the gastric operation was sandwiched from the both sides with two same cylinders, and the incubation medium containing thymidine-H³ was passed from one side with pressure. Three cases of normal fundic gland of human mucosa was examined by this technique. Autoradiograph, Gastric gland : A number of labeled mucosal cell was observed in the neck region of the fundic gland. Labeled mucosal cells were not observed in foveolar, body region or basal region of the gland. The rate of labeling in total cell count was ranged from 9.8 to 19.2%. Lamina mucosa proper : The labeled cells were observed mainly in 1/4 from the surface and a also in 1/4 from the botom of the gastric mucosa in lamina mucosa proper. Salitary lymphonodules : A number of reticular cells was labeled in germinal center. Muscularis mucosa : Few cell was labeled in smooth muscle cells (one cell in a autoradiographic specimen). No histological damage or edama were observed in HE-sained specimens.

ヒト胃粘膜のthymidine-H<SUP>3</SUP>によるautoradiograph圧入によるincubation法について

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