大腸菌およびサルモネラ菌のf<SUP>+</SUP>抗原の血清学的差異について

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タイトル別名
  • SEROLOGICAL DIFFERENCE OF f<SUP>+</SUP> ANTIGENS BETWEEN <I>ESCHERICHIA COLI</I> AND <I>SALMONELLA</I>
  • ダイチョウキン オヨビ サルモネラキン ノ f コウゲン ノ ケッセイガクテキ サイ ニ ツイテ

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Antisera were prepared by injecting Salmonella F+ and Escherichia coli F+ respectively into rabbits, and absorbed with F-, having the same phenotype with F+, to obtain anti-f+ sera, which react with F' strain and Hfr strain. And in this way immunochemical properties of f+ antigens in F' and Hfr strains of Salmonella and Escherichia coli were investigated.<BR>1. Anti-S. f+ antibody reacted with both Escherichia coli Hfr and Salmonella F', but after absorption with the former, remained an antibody which reacted only with latter.<BR>Anti-E. coli f+ antibody reacted with both Salmonella F' and Escherichia coli Hfr, but after absorption with the former, remained an antibody which reacted only with the latter. It is therefore considered that f+ antigens of Salmonella and Escherichia coli contain a common antigen as well as specific antigens, respectively.<BR>2. Bacterial suspensions of Salmonella F' and Escherichia coli Hfr, after treatment with a blendor or a sonicator, were each cetrifuged at 10, 000 r. p. m. and the supernatants were further subjected to ultracentrifugation at 38, 000 r. p. m. The sediments were found to have the highest activity of f+ antigen. This fraction contained about 2% sugar. The f+ antigens found in the living bacterial cells and the ultracetrifuged fraction lost the antigenicity either on heating at 100°C for 60 minutes or on treatment with proteinase such as trypsin, ficin and bromelin. It is subsequently assumed that f+ antigen may have chiefly properties of protein.

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