Hydrolysis of Glucosyl-sucrose and Maltosyl-sucrose in Rat Intestinal Brush Border Membrane

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  • YAMADA Kazuhiko
    Department of Nutrition, School of Health Sciences, Faculty of Medicine, University of Tokyo
  • SASAKI Mitsuyoshi
    Department of Nutrition, School of Health Sciences, Faculty of Medicine, University of Tokyo
  • MORIUCHI Sachiko
    Department of Nutrition, School of Health Sciences, Faculty of Medicine, University of Tokyo
  • HOSOYA Norimasa
    Department of Nutrition, School of Health Sciences, Faculty of Medicine, University of Tokyo

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Other Title
  • 白ネズミ小腸粘膜刷子縁におけるグルコシルスクロースならびにマルトシルスクロースの水解能
  • ハク ネズミ ショウチョウ ネンマク ハケ エン ニ オケル グルコシルクロー

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Description

Hydrolysis of glucosy-sucrose (G2F) and maltosyl-sucrose (G3F) were studied in the brush border of rat intestinal mucosa and compared with those of sucrose and maltose.<BR>Maximum hydrolyzing activities of G2F and G3F were reached at the substrate concentration of 6mM. Optimum pH was 6.0. Hydrolyzing activities of G2F and G3F by intestinal brush border were comparable to that of maltose.<BR>The intial velocity of hydrolysis in the presence of G2F or G3F was less than the sum of the individual rates for G2F and sucrose or G3F and sucrose. This result suggests that there is competition between these two substrates for the same enzyme. Furthermore, the apparent Michaelis constant (Km) and the apparent maximum velocity (Vmax) for pure and mixed substrates, i. e. G2F and sucrose or G3F and sucrose, at various mole fractions (f) of G2F or G3F showed dependence on the mole fraction of G2F or G3F, and provided strong evidence that this competitive inhibition reacts at the same active center of sucrase. Furthermore, the similar results were observed in maltose and G2F or G3F.

Journal

  • Eiyo To Shokuryo

    Eiyo To Shokuryo 31 (3), 267-272, 1978

    JAPAN SOCIETY OF NUTRITION AND FOOD SCIENCE

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