白ネズミ小腸粘膜刷子縁におけるグルコシルスクロースならびにマルトシルスクロースの水解能

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書誌事項

タイトル別名
  • Hydrolysis of Glucosyl-sucrose and Maltosyl-sucrose in Rat Intestinal Brush Border Membrane
  • ハク ネズミ ショウチョウ ネンマク ハケ エン ニ オケル グルコシルクロー

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抄録

Hydrolysis of glucosy-sucrose (G2F) and maltosyl-sucrose (G3F) were studied in the brush border of rat intestinal mucosa and compared with those of sucrose and maltose.<BR>Maximum hydrolyzing activities of G2F and G3F were reached at the substrate concentration of 6mM. Optimum pH was 6.0. Hydrolyzing activities of G2F and G3F by intestinal brush border were comparable to that of maltose.<BR>The intial velocity of hydrolysis in the presence of G2F or G3F was less than the sum of the individual rates for G2F and sucrose or G3F and sucrose. This result suggests that there is competition between these two substrates for the same enzyme. Furthermore, the apparent Michaelis constant (Km) and the apparent maximum velocity (Vmax) for pure and mixed substrates, i. e. G2F and sucrose or G3F and sucrose, at various mole fractions (f) of G2F or G3F showed dependence on the mole fraction of G2F or G3F, and provided strong evidence that this competitive inhibition reacts at the same active center of sucrase. Furthermore, the similar results were observed in maltose and G2F or G3F.

収録刊行物

  • 栄養と食糧

    栄養と食糧 31 (3), 267-272, 1978

    公益社団法人 日本栄養・食糧学会

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