可移植性実験膠腫の電子顯微鏡的研究特に長期累代移植を中心として

DOI Web Site

書誌事項

タイトル別名
  • ELECTRON MICROSCOPIC STUDIES OF TRANSPLANTABLEEXPERIMENTAL MOUSE GLIOMA
  • カ イショクセイ ジッケンコウシュ ノ デンシ ケンビキョウテキ ケンキュウ トクニ チョウキ ルイダイ イショク オ チュウシン ト シテ

この論文をさがす

抄録

The production of experimental tumor was attempted in 60 C57BL mice by giving them20-methylcholanthrene pellets into the cerebrum and the cerebellum. Tumors were produced in 26 animals-glioma in 3 and sarcoma in 23. These 3 cases of glioma, 2 of the 23 cases of sarcoma and other 2 cases of serially transferred glioma (7 cases in total) were cultivated through succesive generations, and cultures of the 1st, 5th, 10th, and 15th generations, were observed by light and electron microscopy. Light microscopically, all the primary gliomas presented a blastoma-like histological picture. Electron microscopically, constituent cells of the transplanted gliomas each had an irregular formed nucleus, and, in the cytoplasm, many ribosomes as well as abundant cytoplasmic organelles such as ergastoplasms and mitochondria. With prolongation of the serial transfer, however, cytoplasmic processes were lost, cell membrane became smooth, and each constituent cell thus came to exhibit a monotonous fine structure. There were scarcely any charactristic features such as gliafilament which indicate gliogenicity and which are foundin normal or reactive astrocytes or in human astrocytoma.<BR>The constituent cell of the transplanted sarcoma, which developed ergastoplasms, distended cisternae and aboundant intercellular collagenous fibers in closè relation with cell membrane, manifested the charactristic feature of sarcoma, clearly distinguishable from that of glioma.In the glioma of serial transfer through mice, round virus-like particles of double shell structure, about 120mμ in diameter, were observed in ergastplasms and other cytoplasmic organelles; they were also seen in spore-like forms on the cell membrane or accumulated in the intercellular space.<BR>It was demonstrated dy electron microscopy as by light microscopy that the trans-plantable experimental glioma consisted of atypical tumor cells poor in gliogenous features

収録刊行物

詳細情報 詳細情報について

問題の指摘

ページトップへ