Quantitative Determination of Polar Lipids on Thin Layer-FID Chromatography

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  • TANAKA Masamichi
    Division of Chemistry, School of General Studies, Kitasato University
  • ITOH Toshihiro
    Division of Chemistry, School of General Studies, Kitasato University
  • KANEKO Hiroshi
    Division of Chemistry, School of General Studies, Kitasato University

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  • シンクログラフィーによる極性脂質の定量に関する研究
  • シンクログラフィー ニヨル キョクセイ シシツ ノ テイリョウ ニカンスルケン

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In the analysis of the phospholipid mixtures which contained phosphatidyicholine, cardiolipin, phosphatidylethanolamine, sphingomyelin and lysophosphatidylcholine, the relative responses for quantitative thin layer chromatography combined with a hydrogen flame ionization detector (thinchrography) represented a good coincidence with the theoretical responses based on weight per cent. <BR>Meanwhile, it was also found that the thinchrograph is a useful tool for the estimation of the relative amounts of nonpolar lipid to polar lipid or neutral lipid to phospholipid and glycolipid in the total lipid extract.

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