Thin-Layer Chromatographic Determination of Neutral Lipids by Photodensitometer
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- JIGAMI Yoshifumi
- National Chemical Laboratory for Industry
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- SUZUKI Osamu
- National Chemical Laboratory for Industry
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- NAKASATO Satoshi
- National Chemical Laboratory for Industry
Bibliographic Information
- Other Title
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- デンシトメータを用いる中性脂質の薄層クロマトグラフィーによる定量
- デンシトメータ オ モチイル チュウセイ シシツ ノ ハクソウ クロマトグラフ
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Abstract
Responses of cholesterol oleate, cholesterol, oleic acid, tri-, di-, and monoolein to the internal standard (methyl oleate) on the TLC plates which were charred after spraying 10% sulfuric acid were determined using a photodensitometer of a zig-zag scanning mode. The relations between the ratio of peak area and that of weight were linear and reproducible over a range of loads (0750μg triolein and 0125μg other lipids) under the same conditions. The responses of cholesterol and its ester, however, were found to be variable by changing charring conditions. The quantitative analysis of yeast neutral lipid revealed that the method was useful and convenient for the estimation of neutral lipid composition indicating approximately 18% of maxium coefficient of variation.
Journal
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- Journal of Japan Oil Chemists' Society
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Journal of Japan Oil Chemists' Society 28 (11), 867-869, 1979
Japan Oil Chemists' Society
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Details 詳細情報について
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- CRID
- 1390282680308903552
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- NII Article ID
- 130001015917
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- NII Book ID
- AN00245435
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- ISSN
- 0513398X
- 18842003
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- NDL BIB ID
- 2070062
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed