ISOLATION AND CHARACTERIZATION OF ANTI-A HEMAGGLUTININ FROM FALCATA JAPONICA

DOI Web Site
  • NAKAJIMA TAMIKO
    Department of Legal Medicine, School of Medicine Gunma University
  • FURUKAWA KEN
    Department of Legal Medicine, School of Medicine Gunma University

Bibliographic Information

Other Title
  • ヤブマメの抗A凝集素の分離精製とその性状に関する研究
  • ヤブマメ ノ コウ A ギョウシュウソ ノ ブンリ セイセイ ト ソノ セイジ

Search this article

Abstract

A 1000-fold purification of anti-A hemagglutinating lectin from Falcata japonica was obtained by means of ammonium sulfate fractionation, gel filtration of Sephadex G-100 and affinity chromatography on N-acetyl-D-galactosamine-starch. The isolated lectin formed a single diffuse band in polyacrylamide gel electrophoresis and has an approximate molecular weight of 120, 000 as estimated by measurements of Sephadex G-200 gel filtration and ultracentrifugal analysis. The lectin was rich in asparatic acid, serine and glycine. Trace of methionine was detected but no cystine was found.

Journal

Details 詳細情報について

Report a problem

Back to top