Automated Colorimetric Method for the Determination of Free Fatty Acids in Fats and Oils and their Derivatives by Auto Analyzer

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  • オートアナライザーによる油脂及び油脂誘導体中の遊離脂肪酸の自動比色定量法
  • オート アナライザー ニ ヨル ユシ オヨビ ユシ ユウドウタイチュウ ノ ユ

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An automated colorimetric method based on continuous flow analysis has been developed for determining free fatty acids in fats and oils and their derivatives.<BR>A 2-propanol solution of a sample was directly introduced into a Technicon Auto Analyzer. o-Nitrophenylhydrazine (ONPH) solution and N-ethyl-N'- [3- (N'', N'' methylamino) propyl] carbodi-imide (EDC) solution containing 5% pyridine were added to the sample solution, followed by heating at 60°C for 12min. The free fatty acids in the sample were converted the corresponding o-nitrophenylhydrazides. The solution was mixed with 1% KOH solution to decrease the absorbance of the reagent blank, followed by measurement at 550nm. The method provides a common molar response to a series of fatty acid from caprylic acid to oleic acid. The sample capacity of themethod is 2040 samples/h. Its reproducibility is within 1% relative and recovery, from of 97100%. It was successfully used for determining lipase activity and quantitating free fatty acids in various fatty acid derivatives.

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