Determination of Sterols in Vegetable Oils by Enzymatic Method Influence of Sterol Structure on the Determination

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  • 酵素法による植物油脂中のステロールの定量ステロール構造の定量値への影響
  • コウソホウ ニ ヨル ショクブツユチュウ ノ ステロール ノ テイリョウ ステ

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The amounts of sterols in vegetable oils were measured by a simple enzymatic method. The applicability of the enzymatic method to determination of various sterols was investigated.<BR>4-Desmethylsterols were oxidized quantitatively by cholesterol oxidase to give satisfactory analytical result. However, 4-mono and 4, 4-dimethylsterols could not be oxidized by cholesterol oxidase. It was also possible to analyze unsaponifiable matter in vegetable oils by this method due to the quite low content of 4-methylsterols.

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