Preparation and Properties of Liposomes Stabilized with Pluronic F-68
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- SASAKI Ichiro
- Research Laboratory, KOSÉ Corporation
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- SUZUKI Tadashi
- Research Laboratory, KOSÉ Corporation
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- ISHII Fumiyoshi
- Meiji College of Pharmacy
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- OGATA Hiroyasu
- Meiji College of Pharmacy
Bibliographic Information
- Other Title
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- Pluronic F-68 を用いた安定なリポソームの調製とその特性
- Pluronic F 68 オ モチイタ アンテイ ナ リポソーム ノ チョウ
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Abstract
Sterically stabilized liposomes were produced by incorporation a poly (oxyethylene) poly (oxypropylene) block copolymer (Pluronic F-68) into the lipid bilayer using a high-pressure homogenizer. Mean vesicle size of liposomes incorporating Pluronic F-68 greatly decreased from 480 nm to 110 nm with minor change in marker entrapment after passing three times through the high-pressure homogenizer. Liposomes stabilized with Pluronic F-68 were characterized with respect to entrapment efficiency, vesicle size and surface charge. Entrapment efficiency of a marker increased with Pluronic F-68 content, and was maximum at 0.13 (% wt/vol) -Pluronic F-68. In particle size and surface charge, no change was noted with increase in Pluronic F-68 content. Surface charge decreased slightly for liposomes incorporating Pluronic F-68, compared to those coated with Pluronic F-68. Pluronic F-68 is thus distributed between the bilayer and/ or inner phase of liposomes as well as the liposome surface. Liposomes stabilized with Pluronic F-68 were significantly stable in calcium and fetal bovine serum (FBS) solution at 37°C compared with Pluronic F-68-free liposomes. The incorporation of Pluronic F-68 into the bilayer caused significant increase in 31P-NMR line width. Pluronic F-68 is thus shown to stabilize liposomes by restricting phospholipid motion in the bilayer.
Journal
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- Journal of Japan Oil Chemists' Society
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Journal of Japan Oil Chemists' Society 43 (7), 567-573, 1994
Japan Oil Chemists' Society
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Details 詳細情報について
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- CRID
- 1390282680311221120
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- NII Article ID
- 10006946267
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- NII Book ID
- AN00245435
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- ISSN
- 0513398X
- 18842003
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- NDL BIB ID
- 3893780
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed