TLC-酵素法による部分加水分解リン脂質中のリゾホスファチジルコリン及びホスファチジルコリンの定量

書誌事項

タイトル別名
  • Determination of Lysophosphatidylcholine and Phosphatidylcholine in Partially Hydrolyzed Phospholipid by TLC-Enzyme Method
  • TLC コウソホウ ニ ヨル ブブン カスイ ブンカイ リン シシツチュウ ノ

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説明

We describe a new method for determination of phosphatidylcholine and lysophosphatidylcholine in partial hydrolyzed phospholipids. The method consists of fractionation of cholinecontaining phospholipid by preparative TLC and enzymatic determination based on the cholineoxidase-phenol method. The method affords better specificity and shorter time than the conventional two-dimensional TLC method are possible. Reproducibility (CV<3 %) and good agreement with those of two-dimensional TLC method were confirmed. This method is thus applicable to the determination of lysophosphatidylcholine and phosphatidylcholine in partially hydrolyzed phospholipids.

収録刊行物

  • 油化学

    油化学 41 (10), 1049-1054, 1992

    公益社団法人 日本油化学会

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