Isolaion and Characterization of Neutral Glycosphingolipidsfrom Annelida (Earthworm, <I>Pheretima hilgendorfi</I>)

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  • SUGITA Mutumi
    Department of Chemistry, Faculty of Liberal Arts and Education, Shiga University
  • HAYATA Chieko
    Department of Chemistry, Faculty of Liberal Arts and Education, Shiga University
  • SUZUKI Minoru
    Department of Membrane Biochemistry, The Tokyo Metropolitan Institute of Medical Science
  • TAKEDA Tadahiro
    Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Nagoya City University
  • MIZUNOMA Tomoko
    Department of Chemistry, Faculty of Liberal Arts and Education, Shiga University
  • MAKINO Tomoka
    Department of Chemistry, Faculty of Liberal Arts and Education, Shiga University
  • NARUSHIMA Kenji
    Department of Chemistry, Faculty of Liberal Arts and Education, Shiga University
  • HORI Taro
    Shiga Junior College of Cultural Studies and Technology

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Other Title
  • 環形動物 (ヒトツモンミミズ<I>Pheretima hilgendorfi</I>) の中性スフィンゴ糖脂質の単離と同定
  • カンケイ ドウブツ ヒトツモンミミズ Pheretima hilgendorfi

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Description

A series of glycosphingolipids containing galactose was purified from the earthworm, Pheretima hilgendorfi by DEAE-and QAE-Sephadex, Florisil, and Iatrobeads column chromatography. From structural studies including compositional sugar analysis, methylation analysis, chromium trioxide oxidation, enzymatic hydrolysis, 1H-and 13C-nuclear magnetic resonance spectroscopy, and fast atom bombardment mass spectrometry, their structures were determined to be as follows : Gal β1-Cer, Gal β1-6 Gal β1-Cer, and Gal β1-6 Gal β1-6 Gal β1-Cer.<BR>The ceramide moiety was composed of octadecasphingenine and its branched homologue as the main sphingoids, and saturated C22-, C23-, and C24-acid as the major fatty acids.<BR>Thin-layer chromatography and the results of sugar analysis indicated the structures of two minor components of the ceramide mono-and trihexoside fractions to be Glc-Cer and Man-Gal-Gal-Cer, respectively.

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