Development of Fluorescent Reagents for Highly Sensitive HPLC

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  • OHRUI Hiroshi
    Department of Biological Chemistry, Faculty of Agriculture, Tohoku University

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  • 蛍光分析試薬の開発とその応用
  • ケイコウ ブンセキ シヤク ノ カイハツ ト ソノ オウヨウ ユカガク ケンキ
  • Analysis and the Application of Them to Lipid Chemistry
  • 油化学研究を中心として

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Description

Several fluorescent analytical reagents for HPLC have been developed. They are 9-BrMa;pmol level determination of carboxylic acids and applied to the routine analysis of 15 common fatty acids in foods, AE-OTf ; amol level determination of carboxylic acids and applied to the determination of DSP-toxins, DPPP ; fmol level determination of hydroperoxides and applied to the determination of lipid hydroperoxides in human plasma, and (+) -TBMB-carboxylic acid ;chiral derivatization reagent for amines and alcohols at fmol level and applied to the D-, L-analysis of amino acids and glycerol derivatives. The problems in this field to be solved in future are also discussed.

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