Specificity of Phospholipase D toward Alcohol Substratesin Transphosphatidylation. II.

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  • ホスファチジル基転移反応におけるホスホリパーゼDのアルコール基質特異性 (第2報)
  • ホスファチジルキ テンイ ハンノウ ニ オケル ホスホリパーゼ D ノ アルコ
  • Effects of Double Bonds or Cyclic Structures in Alcohol Molecules on Conversion Ratios
  • アルコールの二重結合および環構造の変換率に対する影響

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The specificity of phospholipase D prepared from fresh cabbege leaves toward alcohols wasevaluated by transphosphatidylation between phosphatidylcholine (PC) and 15 different alcoholswith double bond or cyclic structures.<BR>Based on the conversion ratios of PC to the corresponding phosphatidylalcohols at less than0.04 M alcohol in the water layer, whose activity was not inhibited by any alcohol, the specificityof phospholipase D was shown not to be affected by double bonds in any alcohol molecule.<BR>The incorporation of cyclic alcohols into PC was more difficult than that of straight chainalcohols, and cyclic alcohols possessing a secondary hydroxyl group were not incorporated atall. That no inositol was incorporated into PC with phospholipase D may thus possible have beendue to inositol's possessing a cyclic structure and no primary hydroxyl group.

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