Determination of Fluorescence Labeled Alcohols from Waxes in Vegetable Oils by High Performance Liquid Chromatography

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  • 蛍光誘導体化による植物油中のワックス構成アルコールのHPLC分析
  • ケイコウ ユウドウタイカ ニ ヨル ショクブツユチュウ ノ ワックス コウセイ

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The authors developed a sensitive and efficient HPLC method for determining alcohol from wax in vegetable oils. Wax isolated by silica gel cartridge was hydrolyzed with alkaline solution followed by 9-anthroyl derivatization. The derivatives separated by a reversed phase HPLC were detected by fluorescence detector. The limit of detection was about 0.1 ng. Under the conditions used phytol, present as an ester, could be determined at the same time. The present method was shown applicable to the determination wax, such as residual wax in dewaxed oil, at low level with satisfactory recovery, repeatability, and sensitivity.

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