Oxidation of Linoleic Acid Catalyzed by Soybean Lipoxygenase I (Optimum pH 9, 0) in Hexane-Water Biphasic System
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- HIRATA Hirofumi
- National Chemical Laboratory for Industry
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- HIGUCHI Katsuhiko
- National Chemical Laboratory for Industry
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- ISHIKAWA Kazuhiko
- National Chemical Laboratory for Industry
Bibliographic Information
- Other Title
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- ヘキサン-水二相系での大豆リポキシゲナーゼI (至適pH9.0) によるリノール酸の酸化反応
- ヘキサン ミズ 2ソウケイ デ ノ ダイズ リポキシゲナーゼ 1 シテキ pH
- Appearance of the Enzyme Activity by Chemical Modification and Addition of Modified Bovine Serum Albumin
- 化学修飾及び修飾した牛血清アルブミンの添加による活性発現
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Abstract
The oxidation of linoleic acid catalyzed by soybean lipoxygenase (LG) I (optimum pH9.0) in a hexane-water biphasic system was studied in response to chemical modification and the use of additives. The amino groups on LG I were acylated by N-acyloxysuccinimides (1) in a phoshate buffer. The modified LG I showed enzyme activity in the biphasic system. In the unmodified form, this activity was slight due to its lack of stability. Efficiency of the modifier for bringing about activity increased with CN (carbon nunber of acyl group) in (1). On the other hand, stability decreased with increasing CN in the modifier. The addition of BSA modified by (1) (CN=6 and 8) also elicited activity in the biphasic system, while additives such as BSA, casein and NaCl had no significant effect. <BR>Based on these observations, a mechanism for the occurrence of LG activity in the biphasic system is proposed and discussed.
Journal
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- Journal of Japan Oil Chemists' Society
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Journal of Japan Oil Chemists' Society 36 (10), 783-790, 1987
Japan Oil Chemists' Society
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Details 詳細情報について
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- CRID
- 1390282680312198400
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- NII Article ID
- 130001019954
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- NII Book ID
- AN00245435
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- ISSN
- 0513398X
- 18842003
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- NDL BIB ID
- 3157185
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed