Modified Method for the Determination of Amino Groups by Sodium 2, 4, 6-Trinitrobenzenesulfonate

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  • 2, 4, 6-トリニトロベンゼンスルホン酸ナトリウムによるアミノ基定量の改良法
  • 2 4 6 トリニトロベンゼンスルホンサン ナトリウム ニ ヨル アミノキ テ

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Abstract

The reactions of amino groups in amino acids and proteins with sodium 2, 4, 6-trinitrobenzenesulfonate (TNBS·Na) in the presence of sodium sulfite (Na2SO3) were studied at pH 10.1. Determination of the amino groups was performed by following the absorbance A (420 nm) of the Meisenheimer complex. A good linear relationship between A and the concentrations of amino compounds was observed. The reactions in a cuvette showed good agreement with those in the dark. Based on these observations, a modified method for determining amino groups by TNBS·Na in the presence of Na2SO3 is proposed, and several comments are described.

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